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8xcts2 mlp eyfp construct  (Addgene inc)


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    Structured Review

    Addgene inc 8xcts2 mlp eyfp construct
    8xcts2 Mlp Eyfp Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/8xcts2+mlp+eyfp+construct/P1-EYFP-pA+(Construct+5)+(Plasmid+%2355197)/pm30940799-202-17-19
    Average 90 stars, based on 3 article reviews
    8xcts2 mlp eyfp construct - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
    Article Snippet: .. To generate the first insert, we first amplified the poly-A signal and pause site out of the 8xCTS2-MLP-EYFP construct (Addgene plasmid # 55197, a gift from Timothy Lu, ref. ) (Oligo Pair 1, Supplementary Table ). .. The amplified fragment was purified by Qiaquick PCR purification (Qiagen) and incubated with five units of standard Taq polymerase in standard Taq buffer with 2.5 mM dATP (NEB) for 15 min at 72 °C to add A overhangs.

    Article Title: Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression.
    Article Snippet: .. To generate the first insert, we first amplified the poly-A signal and pause site out of the 8xCTS2-MLP-EYFP construct (Addgene plasmid # 55197, a gift from Timothy Lu, ref. 19) (Oligo Pair 1, Supplementary Table 1). .. The amplified fragment was purified by Qiaquick PCR purification (Qiagen) and incubated with five units of standard Taq polymerase in standard Taq buffer with 2.5 mM dATP (NEB) for 15 min at 72 °C to add A overhangs.

    Construct:

    Article Title: Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
    Article Snippet: .. To generate the first insert, we first amplified the poly-A signal and pause site out of the 8xCTS2-MLP-EYFP construct (Addgene plasmid # 55197, a gift from Timothy Lu, ref. ) (Oligo Pair 1, Supplementary Table ). .. The amplified fragment was purified by Qiaquick PCR purification (Qiagen) and incubated with five units of standard Taq polymerase in standard Taq buffer with 2.5 mM dATP (NEB) for 15 min at 72 °C to add A overhangs.

    Article Title: Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression.
    Article Snippet: .. To generate the first insert, we first amplified the poly-A signal and pause site out of the 8xCTS2-MLP-EYFP construct (Addgene plasmid # 55197, a gift from Timothy Lu, ref. 19) (Oligo Pair 1, Supplementary Table 1). .. The amplified fragment was purified by Qiaquick PCR purification (Qiagen) and incubated with five units of standard Taq polymerase in standard Taq buffer with 2.5 mM dATP (NEB) for 15 min at 72 °C to add A overhangs.

    Plasmid Preparation:

    Article Title: Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
    Article Snippet: .. To generate the first insert, we first amplified the poly-A signal and pause site out of the 8xCTS2-MLP-EYFP construct (Addgene plasmid # 55197, a gift from Timothy Lu, ref. ) (Oligo Pair 1, Supplementary Table ). .. The amplified fragment was purified by Qiaquick PCR purification (Qiagen) and incubated with five units of standard Taq polymerase in standard Taq buffer with 2.5 mM dATP (NEB) for 15 min at 72 °C to add A overhangs.

    Article Title: Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression.
    Article Snippet: .. To generate the first insert, we first amplified the poly-A signal and pause site out of the 8xCTS2-MLP-EYFP construct (Addgene plasmid # 55197, a gift from Timothy Lu, ref. 19) (Oligo Pair 1, Supplementary Table 1). .. The amplified fragment was purified by Qiaquick PCR purification (Qiagen) and incubated with five units of standard Taq polymerase in standard Taq buffer with 2.5 mM dATP (NEB) for 15 min at 72 °C to add A overhangs.



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    8xcts2 mlp eyfp construct  (Addgene inc)
    90
    Addgene inc 8xcts2 mlp eyfp construct
    8xcts2 Mlp Eyfp Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/8xcts2+mlp+eyfp+construct/P1-EYFP-pA+(Construct+5)+(Plasmid+%2355197)/pm30940799-202-17-19
    Average 90 stars, based on 1 article reviews
    8xcts2 mlp eyfp construct - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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